Cell Migration In Inflammation And Immunity: Methods And Protocols (methods In Molecular Biology)
by Daniele D'Ambrosio /
2003 / English / PDF
3.3 MB Download
Leading cell biologists and immunologists present their most useful
and innovative techniques for studying the molecular and cellular
basis of cell migration in the inflammatory process. The authors
provide a series of focused, cutting-edge techniques proceeding
from the in vitro analysis of cell migration and the molecular
mechanisms underlying this process, to methodologies for the
analysis of cell migration in vivo. Experimental approaches useful
in establishing the role of cell migration in the pathogenesis of
both acute and chronic inflammatory diseases are emphasized.
Methods for the analysis of rapid leukocyte adhesion under flow
conditions in vitro are described, which may prove especially
fruitful for scientists exploring the molecular mechanisms
underlying vascular recognition and leukocyte-endothelium
interaction. Each fully tested protocol includes an introduction
explaining the principle behind the technique, equipment and
reagent lists, and tips on troubleshooting and how to avoid known
pitfalls.
Leading cell biologists and immunologists present their most useful
and innovative techniques for studying the molecular and cellular
basis of cell migration in the inflammatory process. The authors
provide a series of focused, cutting-edge techniques proceeding
from the in vitro analysis of cell migration and the molecular
mechanisms underlying this process, to methodologies for the
analysis of cell migration in vivo. Experimental approaches useful
in establishing the role of cell migration in the pathogenesis of
both acute and chronic inflammatory diseases are emphasized.
Methods for the analysis of rapid leukocyte adhesion under flow
conditions in vitro are described, which may prove especially
fruitful for scientists exploring the molecular mechanisms
underlying vascular recognition and leukocyte-endothelium
interaction. Each fully tested protocol includes an introduction
explaining the principle behind the technique, equipment and
reagent lists, and tips on troubleshooting and how to avoid known
pitfalls.