Applications Of Pcr In Mycology
by Paul Dennis Bridge /
1998 / English / PDF
4.1 MB Download
Since the initial report of the amplification of specific DNA
fragments using the polymerase chain reaction (PCR) in 1985, this
technique has revolutionized molecular biology. It enables the
production of large quantities of DNA from minute amounts of sample
material, which can then be readily analyzed. This facility has had
an enormous influence on the way both fundamental and diagnostic
questions are approached and its use is now considered essential
for molecular work in all branches of biology. The purpose of this
book is to highlight the wide-ranging applications of PCR in pure
and applied mycology and to increase understanding of its potential
benefits. After a brief overview, a group of
internationally-renowned mycologists give definitive descriptions
of the use of PCR in their own specialized fields. These include
fungal gene expression and cloning, taxonomy and speciation, fungal
mycobionts, mycorrhizal fungi, entomopathogenic fungi,
mycotoxin-producing fungi, diagnosis of fungal infections in
animals, seed-borne diseases, fungal/plant interactions and
applications with industrially-important fungi. Finally, potential
future directions for PCR work in mycology are discussed.
Since the initial report of the amplification of specific DNA
fragments using the polymerase chain reaction (PCR) in 1985, this
technique has revolutionized molecular biology. It enables the
production of large quantities of DNA from minute amounts of sample
material, which can then be readily analyzed. This facility has had
an enormous influence on the way both fundamental and diagnostic
questions are approached and its use is now considered essential
for molecular work in all branches of biology. The purpose of this
book is to highlight the wide-ranging applications of PCR in pure
and applied mycology and to increase understanding of its potential
benefits. After a brief overview, a group of
internationally-renowned mycologists give definitive descriptions
of the use of PCR in their own specialized fields. These include
fungal gene expression and cloning, taxonomy and speciation, fungal
mycobionts, mycorrhizal fungi, entomopathogenic fungi,
mycotoxin-producing fungi, diagnosis of fungal infections in
animals, seed-borne diseases, fungal/plant interactions and
applications with industrially-important fungi. Finally, potential
future directions for PCR work in mycology are discussed.
