Bioconjugate Techniques, Second Edition

Bioconjugate Techniques, Second Edition
by Greg T. Hermanson / / / PDF


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Bioconjugate Techniques, 2e provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Bioconjugate Techniques, 2e provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.*A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab, allowing for fast retrieval of tried and tested techniques for synthesizing bioconjugates.

*A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab, allowing for fast retrieval of tried and tested techniques for synthesizing bioconjugates.*Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates.

*Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates.*Includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates, giving researchers a better understanding of how the commercially available reagents facilitate the crosslinking of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

*Includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates, giving researchers a better understanding of how the commercially available reagents facilitate the crosslinking of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.*Includes entirely new chapters on the latest areas in the field of bioconjugation as follows:

*Includes entirely new chapters on the latest areas in the field of bioconjugation as follows:Microparticles and nanoparticles

Microparticles and nanoparticlesSilane coupling agents

Silane coupling agentsDendrimers and dendrons

Dendrimers and dendronsChemoselective ligation

Chemoselective ligationQuantum dots

Quantum dotsLanthanide chelates

Lanthanide chelatesCyanine dyes

Cyanine dyesDiscrete PEG compounds

Discrete PEG compoundsBuckyballs, fullerenes, and carbon nanotubes

Buckyballs, fullerenes, and carbon nanotubesMass tags and isotope tags

Mass tags and isotope tagsBioconjugation in the study of protein interactions

Bioconjugation in the study of protein interactions

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