Cancer Cell Signaling: Methods And Protocols (methods In Molecular Biology)
by David M. Terrian /
2010 / English / PDF
3.3 MB Download
A collection of readily reproducible experimental methods for
studying the subversion of normal cell signaling that leads to
cancer. Presented in step-by-step detail to ensure successful
results, the methods include proven techniques for the
investigation of apoptosis and cell death, complementary protocols
for manipulating and/or monitoring oncogenic signals in cancer
cells, and techniques for studying protein-protein interactions. A
detailed protocol for capturing pure samples of malignant cells
from frozen tissue specimens and two alternative techniques for
analyzing their genomic DNA is also described.
A collection of readily reproducible experimental methods for
studying the subversion of normal cell signaling that leads to
cancer. Presented in step-by-step detail to ensure successful
results, the methods include proven techniques for the
investigation of apoptosis and cell death, complementary protocols
for manipulating and/or monitoring oncogenic signals in cancer
cells, and techniques for studying protein-protein interactions. A
detailed protocol for capturing pure samples of malignant cells
from frozen tissue specimens and two alternative techniques for
analyzing their genomic DNA is also described.
