Cellular Quiescence: Methods And Protocols (methods In Molecular Biology)
by H. Daniel Lacorazza /
2017 / English / PDF
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This detailed volume explores methods and protocols that aim to
increase our understanding of how cells enter a quiescent state
during homeostasis and how cells exit quiescence and re-enter
differentiating cell divisions to restore damaged tissues,
essential for developing new approaches in regenerative medicine
in the future. The chapters in this book were designed to address
cellular quiescence in prokaryote and eukaryote organisms,
detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU,
H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal,
neuronal, hematopoietic), genomic regulation (gene expression,
transcription factors, lncRNA, RNA methylation), as well as
analysis of the heterogeneity of quiescence by computer modeling.
Written for the highly successful
This detailed volume explores methods and protocols that aim to
increase our understanding of how cells enter a quiescent state
during homeostasis and how cells exit quiescence and re-enter
differentiating cell divisions to restore damaged tissues,
essential for developing new approaches in regenerative medicine
in the future. The chapters in this book were designed to address
cellular quiescence in prokaryote and eukaryote organisms,
detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU,
H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal,
neuronal, hematopoietic), genomic regulation (gene expression,
transcription factors, lncRNA, RNA methylation), as well as
analysis of the heterogeneity of quiescence by computer modeling.
Written for the highly successfulMethods in Molecular
Biology
Methods in Molecular
Biology series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.Authoritative and practical,
Authoritative and practical,Cellular Quiescence:
Methods and Protocols
Cellular Quiescence:
Methods and Protocols offers a broad view of basic and
cutting-edge technology to inspire research in this emerging field
of cell biology.
offers a broad view of basic and
cutting-edge technology to inspire research in this emerging field
of cell biology.