Dna Recombination: Methods And Protocols (methods In Molecular Biology)
by Hideo Tsubouchi /
2011 / English / PDF
12.2 MB Download
Homologous recombination is important in various aspects of DNA
metabolism, including damage repair, replication, telomere
maintenance, and meiosis, and yeast genetics has successfully
provided a framework for the mechanism of homologous recombination.
Divided into four convenient sections, DNA Recombination: Methods
and Protocols covers recent techniques that best utilize the
advantages of the yeast system, prescribing to the belief that
yeast will keep serving as a great model organism to study
homologous recombination. Chapters have also been included for such
exceptions as the group of genes involved in recombination that are
found solely in higher eukaryotes, such as BRCA2. And looking
forward, a necessary step in the direction of understanding the
homologous recombination process is to isolate the machine and let
it work in a test tube. Understanding the design by studying
the appearance and behavior of the machinery as a single molecule
will be an important milestone toward understanding the mechanism
of action of the machinery. Techniques covering these topics have
also been included. Written in the successful Methods in
Molecular Biology™ series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible protocols, and notes
on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, DNA Recombination: Methods and
Protocols serves as an ideal guide to scientists of all backgrounds
with its well-honed methodologies and strives to bring the reader
to the next level of understanding regarding this vital subject.
Homologous recombination is important in various aspects of DNA
metabolism, including damage repair, replication, telomere
maintenance, and meiosis, and yeast genetics has successfully
provided a framework for the mechanism of homologous recombination.
Divided into four convenient sections, DNA Recombination: Methods
and Protocols covers recent techniques that best utilize the
advantages of the yeast system, prescribing to the belief that
yeast will keep serving as a great model organism to study
homologous recombination. Chapters have also been included for such
exceptions as the group of genes involved in recombination that are
found solely in higher eukaryotes, such as BRCA2. And looking
forward, a necessary step in the direction of understanding the
homologous recombination process is to isolate the machine and let
it work in a test tube. Understanding the design by studying
the appearance and behavior of the machinery as a single molecule
will be an important milestone toward understanding the mechanism
of action of the machinery. Techniques covering these topics have
also been included. Written in the successful Methods in
Molecular Biology™ series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible protocols, and notes
on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, DNA Recombination: Methods and
Protocols serves as an ideal guide to scientists of all backgrounds
with its well-honed methodologies and strives to bring the reader
to the next level of understanding regarding this vital subject.
