Embryonic Stem Cell Therapy For Osteo-degenerative Diseases: Methods And Protocols (methods In Molecular Biology)
by Nicole I. Nieden /
2010 / English / PDF
7.6 MB Download
Embryonic stem cells (ESCs) offer an unlimited self-renewing
capacity, as opposed to the limits of adult stem cells; therefore,
ESCs represent an almost bottomless resource for regenerative
medicine and tissue engineering approaches. In Embryonic Stem Cell
Therapy for Osteo-Degenerative Diseases: Methods and Protocols,
accomplished investigators provide detailed descriptions on how to
expand ESCs from the most commonly used species ex vivo, i.e. mouse
and human, in static culture as well as in controllable bioreactor
processes. The thorough and timely volume summarizes the methods
that may be used to differentiate these cells along the desired
lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes,
and consequentially also offers analysis tools for the
characterization of resulting cells and evaluation of
differentiation effectiveness. Written in the highly successful
Methods in Molecular Biology™ series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and expert tips on troubleshooting and
avoiding known pitfalls. Dependable and cutting-edge, Embryonic
Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and
Protocols supplies the tools necessary to allow researchers to
carry out critical research needed in order to bring this
burgeoning and vitally important field closer to the clinic and to
ensure the widespread application of a successful strategy.
Embryonic stem cells (ESCs) offer an unlimited self-renewing
capacity, as opposed to the limits of adult stem cells; therefore,
ESCs represent an almost bottomless resource for regenerative
medicine and tissue engineering approaches. In Embryonic Stem Cell
Therapy for Osteo-Degenerative Diseases: Methods and Protocols,
accomplished investigators provide detailed descriptions on how to
expand ESCs from the most commonly used species ex vivo, i.e. mouse
and human, in static culture as well as in controllable bioreactor
processes. The thorough and timely volume summarizes the methods
that may be used to differentiate these cells along the desired
lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes,
and consequentially also offers analysis tools for the
characterization of resulting cells and evaluation of
differentiation effectiveness. Written in the highly successful
Methods in Molecular Biology™ series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and expert tips on troubleshooting and
avoiding known pitfalls. Dependable and cutting-edge, Embryonic
Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and
Protocols supplies the tools necessary to allow researchers to
carry out critical research needed in order to bring this
burgeoning and vitally important field closer to the clinic and to
ensure the widespread application of a successful strategy.
