Erythropoiesis: Methods And Protocols (methods In Molecular Biology)
by Joyce A. Lloyd /
2017 / English / PDF
5.3 MB Download
This detailed volume provides thorough protocols describing how to
use genetics to study mouse and zebrafish erythropoiesis in whole
animal models and for genetically manipulating cultured mouse and
human erythroid cells. Protocols include strategies to analyze
circulating red blood cell parameters, as well as progenitor cell
status, stage of differentiation, and enucleation. Methods for the
study of erythroid cell gene regulation using chromatin
immunoprecipitation and chromatin conformation capture are
detailed. Furthermore, protocols are provided to examine stress
erythropoiesis, erythroblastic islands, and erythroblast structure.
The collection also includes reviews on the available mouse models
of erythropoiesis, as well as on future considerations for good
manufacturing practice in order to translate the manufacture of
erythrocytes to the clinic. Written for the highly successful
This detailed volume provides thorough protocols describing how to
use genetics to study mouse and zebrafish erythropoiesis in whole
animal models and for genetically manipulating cultured mouse and
human erythroid cells. Protocols include strategies to analyze
circulating red blood cell parameters, as well as progenitor cell
status, stage of differentiation, and enucleation. Methods for the
study of erythroid cell gene regulation using chromatin
immunoprecipitation and chromatin conformation capture are
detailed. Furthermore, protocols are provided to examine stress
erythropoiesis, erythroblastic islands, and erythroblast structure.
The collection also includes reviews on the available mouse models
of erythropoiesis, as well as on future considerations for good
manufacturing practice in order to translate the manufacture of
erythrocytes to the clinic. Written for the highly successfulMethods in Molecular Biology
Methods in Molecular Biology series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
Authoritative and practical,
Authoritative and practical,Erythropoiesis: Methods and
Protocols
Erythropoiesis: Methods and
Protocols provides a definitive source for molecular and
cellular biology protocols to study erythropoiesis and to move
this vital field forward.
provides a definitive source for molecular and
cellular biology protocols to study erythropoiesis and to move
this vital field forward.
