Free Radical And Antioxidant Protocols (methods In Molecular Biology)
by Donald Armstrong /
2010 / English / PDF
22 MB Download
This volume presents an outstanding collection of state-of-the-art
methodologies for quantifying free radical and antioxidant analytes
in tissue and body fluids using experimental models and in vitro
procedures. These user-friendly and easily reproducible techniques
cover the essential tasks, including radical generating systems,
direct measurement or trapping of reactive radical species and
acute-phase proteins, and measurement of metabolic intermediates
derived from the oxidation of lipids, proteins, and nucleic acids.
There are also methods for the determination of vitamin, enzymatic,
and water-soluble antioxidants, as well as of essential
micronutrients and cofactors. The techniques take advantage of new
instrumentation and technology-probes, photon counting,
chemiluminescence, and caged compounds, with an emphasis on
HPLC-and are adaptable to a wide range of applications. The
laboratory-tested free-radical assays described here in detail will
illuminate the study of both primary and secondary oxidative stress
and contribute significantly to our understanding of the many
disorders associated with this process.
This volume presents an outstanding collection of state-of-the-art
methodologies for quantifying free radical and antioxidant analytes
in tissue and body fluids using experimental models and in vitro
procedures. These user-friendly and easily reproducible techniques
cover the essential tasks, including radical generating systems,
direct measurement or trapping of reactive radical species and
acute-phase proteins, and measurement of metabolic intermediates
derived from the oxidation of lipids, proteins, and nucleic acids.
There are also methods for the determination of vitamin, enzymatic,
and water-soluble antioxidants, as well as of essential
micronutrients and cofactors. The techniques take advantage of new
instrumentation and technology-probes, photon counting,
chemiluminescence, and caged compounds, with an emphasis on
HPLC-and are adaptable to a wide range of applications. The
laboratory-tested free-radical assays described here in detail will
illuminate the study of both primary and secondary oxidative stress
and contribute significantly to our understanding of the many
disorders associated with this process.
