Hemorrhagic Fever Viruses: Methods And Protocols (methods In Molecular Biology)
by Maria S. Salvato /
2017 / English / PDF
8.9 MB Download
This volume presents protocols that analyze and explore hemorrhagic
fever viruses (HFV). This book is divided into 5 parts: Part I
begins with an overview on predicting viral pandemics and then
covers methods for surveillance, diagnosis, and classification of
HFV. This includes an antibody capture method using Lassa virus
antigens. Part II discusses structural studies and reverse genetics
of HFV. The chapters in this part describe envelope glycoprotein
membrane fusion studies, arenavirus nucleocapsid protein, and the
use of virus-like-particles to study viral egress. Part III
explores in vivo models of HFV infections, and contains chapters on
murine, guinea pig, and primate models for HFV, and methods to
obtain a subset of primary human liver cells that can be cultured
long-term. Part IV looks into immune assays and vaccine production
for HFV. The chapters in this section cover the attenuated vaccine
for Argentine HFV, detecting virus-antibody immune complexes in
secondary dengue infections, and DNA vaccination. Part V discusses
host responses to viral hemorrhagic fever, and contains chapters on
identifying host restrictions to Junín or Dengue infection, and a
cell-culture method to assess coagulation after HFV infection.
Written in the highly successful
This volume presents protocols that analyze and explore hemorrhagic
fever viruses (HFV). This book is divided into 5 parts: Part I
begins with an overview on predicting viral pandemics and then
covers methods for surveillance, diagnosis, and classification of
HFV. This includes an antibody capture method using Lassa virus
antigens. Part II discusses structural studies and reverse genetics
of HFV. The chapters in this part describe envelope glycoprotein
membrane fusion studies, arenavirus nucleocapsid protein, and the
use of virus-like-particles to study viral egress. Part III
explores in vivo models of HFV infections, and contains chapters on
murine, guinea pig, and primate models for HFV, and methods to
obtain a subset of primary human liver cells that can be cultured
long-term. Part IV looks into immune assays and vaccine production
for HFV. The chapters in this section cover the attenuated vaccine
for Argentine HFV, detecting virus-antibody immune complexes in
secondary dengue infections, and DNA vaccination. Part V discusses
host responses to viral hemorrhagic fever, and contains chapters on
identifying host restrictions to Junín or Dengue infection, and a
cell-culture method to assess coagulation after HFV infection.
Written in the highly successfulMethods in Molecular
Biology
Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge,
Thorough and cutting-edge,Hemorrhagic Fever Viruses: Methods
and Protocols
Hemorrhagic Fever Viruses: Methods
and Protocols is a valuable resource for scientists and
researchers who want to bridge the gap between virus recognition in
surveillance and understanding host responses to infection.
is a valuable resource for scientists and
researchers who want to bridge the gap between virus recognition in
surveillance and understanding host responses to infection.