Lymphocytes: A Practical Approach (practical Approach Series)
by Sarah L. Rowland-Jones /
2000 / English / PDF
18.2 MB Download
Cellular immunology is a rapidly moving field in which recent
advances have made significant contributions to our understanding
of the immune response to infection and malignancy. These in turn,
have given rise to new therapeutic opportunities in areas such as
vaccines and immunotheraphy. Many investigators have been
discourages by the complicated protocols involved in cellular
immunological studies, as illustrated, by the meticulous care
required for the generation of antigen-specific T-cells.
Cellular immunology is a rapidly moving field in which recent
advances have made significant contributions to our understanding
of the immune response to infection and malignancy. These in turn,
have given rise to new therapeutic opportunities in areas such as
vaccines and immunotheraphy. Many investigators have been
discourages by the complicated protocols involved in cellular
immunological studies, as illustrated, by the meticulous care
required for the generation of antigen-specific T-cells.Lymphocytes: A Practical Approach
Lymphocytes: A Practical Approach (second edition)
contains straight-forward protocols for well- established
procedures in the study of lymphocytes including preparation and
identification of lymphocytes, immortalization, cell and organ
culture, and quantification assays. It also covers the recent
technological advances which have revolutionised the field, such as
the use of the Interferon-gamma ELISpot assay and peptide-HLA
tetrameric assays to quantify antigen-specifidc T-cells directly
from peripheral blood, without the need for
(second edition)
contains straight-forward protocols for well- established
procedures in the study of lymphocytes including preparation and
identification of lymphocytes, immortalization, cell and organ
culture, and quantification assays. It also covers the recent
technological advances which have revolutionised the field, such as
the use of the Interferon-gamma ELISpot assay and peptide-HLA
tetrameric assays to quantify antigen-specifidc T-cells directly
from peripheral blood, without the need forin vitro
in vitro
culture, and molecular methods for accurate HLA typing.
culture, and molecular methods for accurate HLA typing.
