Metabolic Flux Analysis In Eukaryotic Cells: Methods And Protocols
by Deepak Nagrath /
2020 / English / PDF
9.4 MB Download
This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing isotopologue fractions using GC-TOF non-targeted mass isotopolome analysis large-scale profiling of cellular metabolic activities using deep 13C labeling medium metastases in mice SWATH Exo-MFA metabolic flux from time-course metabolomics and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.
