Microsatellites: Methods And Protocols (methods In Molecular Biology)
by Stella K. Kantartzi /
2013 / English / PDF
6.3 MB Download
Microsatellites or simple sequence repeats (SSRs) have become the
markers of choice for a variety of molecular studies because of
their versatility, operational flexibility, and lower cost than
other marker systems. Microsatellites: Methods and Protocols
brings together experts in the field to cover this significant area
of research. Broken in to four convenient parts, this volume
delves into classical and modern methods for the discovery and
development of microsatellite markers, descriptions of
amplification and visualization of SSRs, automated capillary
sequencers that are widely used for fragment analysis, as well as a
variety of methods for the analysis of data obtained by the use of
microsatellites. Written for the highly successful Methods in
Molecular Biology™ series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, Microsatellites: Methods and
Protocols aims at researchers that need detailed protocols for
incorporating microsatellite markers into their projects and expert
scientists looking to expand their knowledge of SSRs discovery,
use, and analysis.
Microsatellites or simple sequence repeats (SSRs) have become the
markers of choice for a variety of molecular studies because of
their versatility, operational flexibility, and lower cost than
other marker systems. Microsatellites: Methods and Protocols
brings together experts in the field to cover this significant area
of research. Broken in to four convenient parts, this volume
delves into classical and modern methods for the discovery and
development of microsatellite markers, descriptions of
amplification and visualization of SSRs, automated capillary
sequencers that are widely used for fragment analysis, as well as a
variety of methods for the analysis of data obtained by the use of
microsatellites. Written for the highly successful Methods in
Molecular Biology™ series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, Microsatellites: Methods and
Protocols aims at researchers that need detailed protocols for
incorporating microsatellite markers into their projects and expert
scientists looking to expand their knowledge of SSRs discovery,
use, and analysis.