Oxidative Stress Biomarkers And Antioxidant Protocols (methods In Molecular Biology)
by Donald Armstrong /
2002 / English / PDF
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Donald Armstrong updates and expands his highly praised Free
Radical and Antioxidant Protocols (Humana, 1998) with a collection
of new and valuable methods for evaluating the pertubations in cell
function resulting from increased oxidative stress. Presented in a
user-friendly, step-by-step format, these readily reproducible
techniques cover both free radical-derived and antioxidant
biomarkers. The methodologies demonstrated include ELISA, HPLC,
infrared spectroscopy, gas chromatography-mass spectroscopy,
immunoblotting, electroelution fractionation, isoelectric focusing,
voltametry, and electron paramagnetic resonance imaging. Special
emphasis is given to the separation of complex mixtures of plant
antioxidants, soft drug design to protect from toxic oxidative
metabolites, in vitro oxidation conditions, and correcting for
random measurement error to improve statistical interpretation. A
soon-to-be-published companion volume, Oxidants and Antioxidants:
Ultrastructural and Molecular Biology Protocols (0-89603-851-3),
contains state-of-the-art molecular and ultrastructural methods
that expand the total number of protocols to 109 assays.
Donald Armstrong updates and expands his highly praised Free
Radical and Antioxidant Protocols (Humana, 1998) with a collection
of new and valuable methods for evaluating the pertubations in cell
function resulting from increased oxidative stress. Presented in a
user-friendly, step-by-step format, these readily reproducible
techniques cover both free radical-derived and antioxidant
biomarkers. The methodologies demonstrated include ELISA, HPLC,
infrared spectroscopy, gas chromatography-mass spectroscopy,
immunoblotting, electroelution fractionation, isoelectric focusing,
voltametry, and electron paramagnetic resonance imaging. Special
emphasis is given to the separation of complex mixtures of plant
antioxidants, soft drug design to protect from toxic oxidative
metabolites, in vitro oxidation conditions, and correcting for
random measurement error to improve statistical interpretation. A
soon-to-be-published companion volume, Oxidants and Antioxidants:
Ultrastructural and Molecular Biology Protocols (0-89603-851-3),
contains state-of-the-art molecular and ultrastructural methods
that expand the total number of protocols to 109 assays.
