Plant Cell Culture Protocols (methods In Molecular Biology)
by Víctor M. Loyola-Vargas /
2012 / English / PDF
6.4 MB Download
Cell culture methodologies have become standard procedures in most
plant laboratories. Currently, facilities for in vitro cell
cultures are found in practically every plant biology laboratory,
serving different purposes since tissue culture has turned into a
basic asset for modern biotechnology, from the fundamental
biochemical aspects to the massive propagation of selected
individuals. “Plant Cell Culture Protocols, Third Edition is
divided into five convenient sections that cover topics from
general methodologies, such as culture induction, growth and
viability evaluation, statistical analysis and contamination
control, to highly specialized techniques, such as clonal
propagation, haploid production, somatic embryogenesis, organelle
transformation. The volume concludes with a section on the
laborious process of measuring the epigenetics changes in tissue
cultures.”Written in the successful Methods in Molecular Biology™
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative
and easily accessible, Plant Cell Culture Protocols, Third Edition
seeks to serve both professionals and novices with its guide to the
most common and applicable techniques and methods for plant tissue
and cell culture.
Cell culture methodologies have become standard procedures in most
plant laboratories. Currently, facilities for in vitro cell
cultures are found in practically every plant biology laboratory,
serving different purposes since tissue culture has turned into a
basic asset for modern biotechnology, from the fundamental
biochemical aspects to the massive propagation of selected
individuals. “Plant Cell Culture Protocols, Third Edition is
divided into five convenient sections that cover topics from
general methodologies, such as culture induction, growth and
viability evaluation, statistical analysis and contamination
control, to highly specialized techniques, such as clonal
propagation, haploid production, somatic embryogenesis, organelle
transformation. The volume concludes with a section on the
laborious process of measuring the epigenetics changes in tissue
cultures.”Written in the successful Methods in Molecular Biology™
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative
and easily accessible, Plant Cell Culture Protocols, Third Edition
seeks to serve both professionals and novices with its guide to the
most common and applicable techniques and methods for plant tissue
and cell culture.
