Plant Kinases: Methods And Protocols (methods In Molecular Biology)
by Nico Dissmeyer /
2011 / English / PDF
4.9 MB Download
Modification of target protein properties by reversible
phosphorylation events has been found to be one of the most
prominent cellular control processes in all organisms. Recent
advances in the areas of molecular biology and biochemistry are
presenting new possibilities for reaching an unprecedented depth
and a proteome-wide understanding of phosphorylation processes in
plants as well as in other species. The major goal of Plant
Kinases: Methods and Protocols is to provide the experimentalist
with a detailed account of the practical steps necessary for
successfully carrying out each protocol in his or her own
laboratory. Plant protein kinases specifically addressed in this
volume are members of the plant MAP kinase cascade, cyclin- and
Calcium-dependent protein kinases, and plant sensor and receptor
kinases. Written in the highly successful Methods in Molecular
Biology™ series format, chapters contain introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Kinases: Methods and Protocols
will prove a useful laboratory companion to both novice and
seasoned researchers by facilitating the practical work that will
lead them to new and exciting insights in this dynamic field.
Modification of target protein properties by reversible
phosphorylation events has been found to be one of the most
prominent cellular control processes in all organisms. Recent
advances in the areas of molecular biology and biochemistry are
presenting new possibilities for reaching an unprecedented depth
and a proteome-wide understanding of phosphorylation processes in
plants as well as in other species. The major goal of Plant
Kinases: Methods and Protocols is to provide the experimentalist
with a detailed account of the practical steps necessary for
successfully carrying out each protocol in his or her own
laboratory. Plant protein kinases specifically addressed in this
volume are members of the plant MAP kinase cascade, cyclin- and
Calcium-dependent protein kinases, and plant sensor and receptor
kinases. Written in the highly successful Methods in Molecular
Biology™ series format, chapters contain introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Kinases: Methods and Protocols
will prove a useful laboratory companion to both novice and
seasoned researchers by facilitating the practical work that will
lead them to new and exciting insights in this dynamic field.
