Transcriptional Regulation In Eukaryotes: Concepts, Strategies And Techniques
by Michael Carey /
2000 / English / PDF
7.9 MB Download
After a review of the state-of-the-art in the RNA polymerase II
transcription field, subsequent chapters present a systematic guide
to the investigation of regulatory mechanisms for newly identified
genes, and discuss approaches for synthesizing recombinant
transcription factors, analyzing their binding, and deciphering
their mode of action. Carey (UCLA) and Smale (Howard Huges Medical
Institute and UCLA) write for graduate students,
physician-scientists, and others, either beginning or already
established, who are undertaking in-depth studies in the analysis
of gene expression in model systems or disease states. They put
methods in the context of underlying theory, recommend experimental
strategies, encourage creativity in investigative design, and
explain protocols, with troubleshooting advice.
After a review of the state-of-the-art in the RNA polymerase II
transcription field, subsequent chapters present a systematic guide
to the investigation of regulatory mechanisms for newly identified
genes, and discuss approaches for synthesizing recombinant
transcription factors, analyzing their binding, and deciphering
their mode of action. Carey (UCLA) and Smale (Howard Huges Medical
Institute and UCLA) write for graduate students,
physician-scientists, and others, either beginning or already
established, who are undertaking in-depth studies in the analysis
of gene expression in model systems or disease states. They put
methods in the context of underlying theory, recommend experimental
strategies, encourage creativity in investigative design, and
explain protocols, with troubleshooting advice.
