Pcr Mutation Detection Protocols (methods In Molecular Biology)
by Ralph Rapley /
2010 / English / PDF
6.2 MB Download
Since the publication of the popular first edition, the explosion
of DNA sequence information, the access to bioinformatics and
mutation databases coupled with the ability to readily detect and
confirm mutations has cemented the role of molecular diagnostics in
medicine and, in particular, mutation detection by the polymerase
chain reaction (PCR). In PCR Mutation Detection Protocols, Second
Edition, expert researchers bring the subject up-to-date with key
protocols involving the PCR and its many various incarnations such
as SSCP, CSGE, and dHPLC. The volume also addresses key areas such
as Southern blotting, accurate diagnostics with high throughput, as
well as microarray systems. Written in the highly successful
Methods in Molecular Biology™ series format, chapters include brief
introductions their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes which provide the often hard to
find information that may mean the difference between the success
and failure of the method. Authoritative and cutting-edge, PCR
Mutation Detection Protocols, Second Edition aims to stimulate
postgraduate scientists, researchers, and clinicians already
engaged in the area and to provide an important first step for
those new to this practice wanting to adopt the powerful and
essential technique in their own laboratories.
Since the publication of the popular first edition, the explosion
of DNA sequence information, the access to bioinformatics and
mutation databases coupled with the ability to readily detect and
confirm mutations has cemented the role of molecular diagnostics in
medicine and, in particular, mutation detection by the polymerase
chain reaction (PCR). In PCR Mutation Detection Protocols, Second
Edition, expert researchers bring the subject up-to-date with key
protocols involving the PCR and its many various incarnations such
as SSCP, CSGE, and dHPLC. The volume also addresses key areas such
as Southern blotting, accurate diagnostics with high throughput, as
well as microarray systems. Written in the highly successful
Methods in Molecular Biology™ series format, chapters include brief
introductions their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes which provide the often hard to
find information that may mean the difference between the success
and failure of the method. Authoritative and cutting-edge, PCR
Mutation Detection Protocols, Second Edition aims to stimulate
postgraduate scientists, researchers, and clinicians already
engaged in the area and to provide an important first step for
those new to this practice wanting to adopt the powerful and
essential technique in their own laboratories.